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Capillary-loaded particle fluid dynamics: effect on estimation of sperm concentration.

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Description:This SS effect depends on the transverse velocity gradient in the laminar flow. In computer-automated semen analysis, a standard microm x mm x 6-mm chamber is commonly used, and these studies supported our previously published fluid-flow theory for this type of chamber. Poiseuille flow of specimen into these chambers causes migration of suspended particles or cells in a direction transverse to the flow, which results in their preferential accumulation in the Segre-Silberberg SS planes. Conversely, the SS effect does not appear to have time to develop in the microm-depth hemacytometer, which is deeper than the standard slide, has lower transverse velocity gradient, and consequently does not exhibit concentration variation due to the SS effect. Abstract Capillary loaded chambers are frequently used for semen analysis. These studies compared diluted boar semen concentrations estimated by standard hemacytometry and in capillary-loaded microm slides, using a computer-automated semen-analysis system designed to compensate for the SS effect. Applicability of our findings to routine semen analyses was then tested in 2 studies performed with independent boar studs.

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